Translational_Unit
rbs+ftsK

Part:BBa_I742164

Designed by: Caroline Dahl and Xiaonan Wang   Group: iGEM07_Edinburgh   (2007-10-25)

ftsK (cell division protein) of E. coli with native rbs

This is cell division gene ftsK of Escherichia coli JM109 (a K12 strain) with its native ribosome binding site. Note that this is the native sequence with a TTG start codon and two internal PstI sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1192
    Illegal PstI site found at 1255
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 755
    Illegal PstI site found at 1192
    Illegal PstI site found at 1255
    Illegal NotI site found at 79
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3821
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1192
    Illegal PstI site found at 1255
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1192
    Illegal PstI site found at 1255
    Illegal AgeI site found at 561
    Illegal AgeI site found at 1307
    Illegal AgeI site found at 1583
    Illegal AgeI site found at 2258
    Illegal AgeI site found at 2375
    Illegal AgeI site found at 2414
    Illegal AgeI site found at 3003
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1335
    Illegal SapI.rc site found at 3429


[edit]
Categories
//classic/generator/RC
Parameters
None